ruo viral vector
services
Over 20 years of expertise in viral vector production, providing gene delivery systems for your research success.
Ready to take your research projects to new heights? Choose excellence with our standard or customized gene delivery solutions. With our well-established viral vector production process and experienced experts by your side every step of the way, we ensure optimized viral transduction of your cells.
HIGHLIGHTS OF OUR SERVICES
Track record in vector production
11 000+ batches manufactured
3 weeks
turnaround time
One
stop shop
More than 2000 global clients are trusting us
LentiCare® grades
FlashRNA® grades
lentiVIRAL VECTOR GRADES
Discover our range of integrative viral vector services tailored to meet your needs: LentiCare® technology.
To address your specific needs, we have tailored our production and purification process to offer you 3 types of LentiCare® batches, each designed to perfectly suit the applications of your projects:
- Ready range
- Start range
- Premium range
Ready
Volume
≥ 10 mL
Titer by qPCR
≥ 106 IG/mL
PP/IG
<1000
Application
Screening
Turnaround Time
3 weeks
Start
Volume
≥ 1 mL
Titer by qPCR
≥ 107 IG/mL
PP/IG
<1000
Applications
Screening
Transduction and generation of stable cell lines
Turnaround Time
3 weeks
Premium
Volume
≥ 1 mL
Titer by qPCR
≥ 109 IG/mL
PP/IG
<300
Applications
Ex vivo approaches
In vivo gene therapy
Transgenesis or direct tissue injection experiments
Turnaround Time
3 weeks
flashrna® particles GRADES
Discover our range of RNA delivery solutions tailored to meet your needs: FlashRNA® technology.
To cater to your specific needs, we have tailored our production and purification process to provide you with 2 types of FlashRNA® batches, each ideally suited to the requirements of your projects:
- Start range
- Premium range
Start
Volume
≥ 1 mL
Titer by ELISA
≥ 109 PP/mL
Applications
In vitro cell transduction (cell lines and permissive primary cells)
Turnaround Time
4 weeks
Premium
Volume
≥ 1 mL
Titer by ELISA
≥ 1011 PP/mL
Applications
In vivo, in vitro and ex vivo cell transduction (including non-permissive primary and stem cells)
Turnaround Time
4 weeks
Recombinant viral vector services
Recombinant lentiviral vectors are powerful tools for delivering genes into mammalian cells. We have developed an optimized lentiviral system with the highest safety features. Our vector packaging services involve co-transfection of the transfer plasmid containing the gene of interest (GOI), the envelope plasmid (classically expressing VSV-G), and the packaging plasmid(s) into producer cells.
Once produced, viral vectors are highly purified and concentrated enabling safe use for in vitro and in vivo research applications.
We understand the crucial importance of reliable and safe viral vector production. That’s why we have optimized our protocols for manufacturing high-quality recombinant viral vectors to enhance titer, purity, and efficacy.
Robust and well-established bioproduction process
QC and robust titration methods
We’ve developed a purification process for the production of high-purity, high-titer viral vectors that comply with cGMP standards, ensuring that residual protein and DNA impurity levels meet the drug product specifications. This ensures optimal preservation of transduced cell phenotypic profiles and cellular responses.
At Flash BioSolutions, each construct undergoes rigorous quality control procedures, reflecting our commitment to excellence. We meticulously measure the functional titers of viral vectors, provided in Integrated Genomes per milliliter (IG/ml), by using qPCR to specifically target a unique sequence in the vector genome, ensuring unparalleled accuracy. This precise methodology provides a clear view of efficient particles, thereby delivering the real number of integrative particles crucial for the success of your research projects.
For more in-depth quality control needs in your research projects, we also offer additional analyses and titration methods such as the p24 ELISA assay, giving titers in Physical Particles per milliliter (PP/mL) and the Transgene Expression Assay (TU/mL), see table below.
| Standard QC Methods | Description | Viral titers specifications for a Premium batch |
|---|---|---|
| Titration by ELISA (PP/mL) | Physical particles : Quantification of viral structure element (p24 viral capsid protein) directly in the viral supernatant by ELISA, taking into account functionnal and non-functionnal vector particles. | > 1E11 PP/mL |
| Titration by qPCR (IG/mL) | Functional viral titer : Quantification of the number of integrated genomes by qPCR (true count of functional particles) and assessment of their integration capacity. | > 1E9 IG/mL |
| Transgene Expression Assay (TU/mL) | Specified according to your needs : Accurate assessment of the dose effect linked to the expression of transgenres of interest, by direct detection of fluorescent reporters or by specific immunostaining using flow cytrometry. | > 1E9 TU/mL |
Standard
viral vectors
Ready-to-use viral vectors guaranteeing up to 100% transduction efficiency on primary cells, stem cells and cell lines.
Custom
viral vectors
Tailor-made viral vectors are perfectly fitted to your requirements
CONTACT US
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+33(0) 5 61 28 70 75