DESIGN MY viral vector
Our custom viral vector production service enables you to personaliZe your research project by following a 5-step process.
This service includes plasmid design, molecular cloning, plasmid production, viral vector production, vector purification and titration. As an option, we also propose gene synthesis and the addition of reporters or selection markers.
Flash BioSolutions also offers a range of control vectors, including fluorescent and bioluminescent reporter-expressing vectors, negative control, and a host of other products.
Custom your plasmid vector construction
For full details of the information to be provided, please consult the section below the form.
During all the process you will be in close collaboration with all our team of experts. For any assistance, please feel free to contact our team, who will be happy to answer your questions.
*For instance, if the available vector options do not include the desired promoter, selection marker, or tag, or if you have any other special requests, kindly inform us.
More details
Vector backbone
| Characteristics | LentiCare® viral vector | FlashRNA® |
|---|---|---|
| Delivery technology | DNA delivery | RNA delivery |
| Integration | Integrating | Non-integrating |
| Expression | Long terme & Stable | Short term & Transient |
| Payload | 10 kB | 10 kB |
| Infection | Dividing and non-dividing cells | Dividing and non-dividing cells |
| Ex vivo efficiency | High | High |
| Immunogenicity | Very low | Very low |
| GMO generation | Yes | No |
Promoter Specification
Explore the table below to discover which of our promoters could best fit with your experiments fo a maximun success. Can’t find what you need? Additional promoters are available on demand.
| Required specificity of expression | Recommended promoters |
|---|---|
| Ubiquitous | EF1a, PGK |
| Inducible | TRE3G |
| Liver | APOA2 |
| Pancreas | INS |
| Central Nervous System | SYN (neurons), GFAP (astrocytes), MBP, TH |
| Heart | MYL2, MYH6 |
| Endothelial cells | ENG |
| Cell senescence | p21, p16, p53RE |
| Inflammatory response | NFkappaB-RE |
| Cell proliferation | MKI67, PCNA |
Add an ORF (option)
Seeking personalized viral vectors? Take advantage of our gene synthesis and mutagenesis services for tailored orders. Have a specific sequence in mind? Feel free to provide it, and we’ll subclone it into a custom viral vector transfer plasmid, ensuring all necessary details are provided.
Add a Tag, a Reporter and/or a Selection marker (option)
Choose from a variety of reporters and selection markers to customize our viral vectors during molecular cloning. Add the tag of your choice and specify your preference for N- or C-terminus fusion to your gene of interest when requesting a quote, and we’ll tailor the vector to your needs.
| Genetic elements | Available options |
|---|---|
| Tags | Any tags available to customize your plasmid to your needs. |
| Reporters | mCherry, ZsGreen, turboGFP, turboRFP, mKate, Luc or any reporter of your choice |
| Selection markers | Puromycin (Puro), Neomycin (Neo), Hygromycin B (Hygro), Blasticidin (Bsd), Zeomycin (Zeo) |
Purity level according to range
- VectaReady (106 IG/mL)
- VectaStart (107 IG/mL)
- VectaPremium (109 IG/mL)
Standard viral vectors
Standard viral vectors can achieve up to 100% transduction efficiency on primary cells, stem cells and cell lines.